Samuel married Anna "Ann" Marshall in 1730 in Maherrin River, Brunswick, Virginia, Colonial America. Anna was born on 28 May 1712 in Brunswick County, Virginia, USA; died in 1783 in Warren County, North Carolina, USA. [Group Sheet] [Family Chart]

1. Ann Harrison Grove was born in 1645; died in Abt. 1716.
2. Benjamin Despain was born in 1720 in Leonards, London, England; died on 19 Apr 1727 in London, London, England.
3. Susan Despain was born between 1730 and 1770.
4. Lewis Despain was born in Bet. 1730–1770.
5. Mark Despain was born in Bet. 1730–1770.
6. John DeSpain was born in 1735 in Maherrin River, Brunswick, Virginia, United States; died in 1806 in Brush Creek, Green, Kentucky, United States.