Leishmania mexicana isolates from 23 patients with localized, scattered, and an irregular “pseudodiffuse” type of cutaneous leishmaniasis were obtained in various endemic areas of Mexico. Restriction fragment length polymorphism analysis of kinetoplast DNA was made with 9 different endonucleases in addition to an in vitro development pattern analysis. We found that the 23 L. mexicana isolates could be regularly classified into six groups, according to the endonuclease food digestion patterns acquired with HaeIII, HpaII, and MseI. Whereas localized cutaneous leishmaniasis isolates might have any of five patterns, diffuse cutaneous leishmaniasis showed just two patterns and pseudodiffuse cutaneous leishmaniasis regularly showed just one pattern. Hence, a clear connection amongst digestion pattern, scientific illness, and geographical localization was gotten for the pseudodiffuse cutaneous leishmaniasis group. Furthermore, the L. mexicana isolates could be separated into quick- and slow-growing groups. Diffuse cutaneous leishmaniasis isolates were discovered to be fast growing, whereas localized cutaneous leishmaniasis isolates fell into both categories. On the other hand, all pseudo scattered cutaneous leishmaniasis isolates were sluggish growing. Here we report the first research study in which distinct and consistent genotypic qualities of kinetoplast DNA heterogeneity within the L. mexicana species could be directly correlated with clinical illness and its growth habits, recommending that a distinctive limitation pattern might have crucial biological ramifications. In addition, this research study sheds brand-new light on the biological significance of parasite kinetoplast DNA, given that the heterogeneity seems not to be random but to form a distinct pattern.